New England Biolabs has introduced a line of restriction enzymes that has been engineered for reduced star activity. Star activity (relaxed specificity) can be problematic for some enzymes when setting up reactions with extended incubation times, smaller reaction volumes or increased number of units. The enzymes perform under a range of reaction conditions. They are supplied with NEBuffer 4, which may simplify double digest reaction and are available for the same price as the wild type enzyme. New England Biolabs, Inc., 800-632-5227, www.neb.com.