Adiponectin (Acrp30), an adipocyte-specific secreted protein, circulates in plasma and plays a role in various physiological processes, such as energy homeostasis and obesity. Active recombinant human, mouse, and rat adiponectin was expressed using perspective adiponectin of cDNA.
Upstate offers a rapid and sensitive microplate-based assay for the detection of H2A.X phosphorylation. Histone H2A.
The KODAK Image Station 2000 Multi-Modal Imaging System (IS2000MM) utilizes a cooled CCD camera to provide comprehensive fluorescence, luminescence, radiographic and chromogenic imaging.
The CelljecT Uno electroporator is a compact unit designed originally for bacterial applications. A new set of protocols for yeast applications greatly expands the role of this unit. These protocols are supplied with every new unit, along with a free 40-page optimization guide.
The BTX ECM 830 electroporator was cited in "RNA Interference Shows Critical Requirement for NF-?B p50 in the Production of IL-12 by Human Dendritic Cells," (Laderach, D., Campagno, D., Danos, O., Vainchenker, W. Galy, A. ( J. Immunology, 171:1750-1757 (2003).
MicroPulser electroporator enables safe and reproducible transformation of bacteria, yeast, and other microorganisms. The system offers one-button pulse delivery, attached cuvette chamber, and rapid charge-time speed sample handling.
Cloning Gun cordless, rechargeable, hand-held electroporation instrument achieves transfection efficiencies exceeding 50% of viable cells with a variety of standard mammalian cell lines, and Transformation Efficiencies (TE's) of over 10 10 transformants per μg of input DNA with E.
Electroporation cuvettes are offered in three color-coded gap sizes suitable for electroporation of bacteria, yeast or mammalian cells. The cuvettes fit most brands of electroporators and are individually wrapped and gamma irradiated for sterility.
A hybrid mass spectrometer has reduced the time needed to analyze metabolites of drug compounds by two-thirds or more, according to researchers at the University of Geneva.
OriGene's TrueClone Collection consists of over 20,000 full-length human cDNA clones. Each clone is in expression-ready vector and is suitable for transfection in mammalian cells and for direct in vitro expression.
USB Corporation reagents for various cloning applications include Tested User Friendly (TUF) enzymes. T4 DNA Ligase is available in both low and high concentrations, allowing flexibility in ligation reactions.
Lucigen's PCR-SMART Cloning Kits incorporate the company's CloneSmart technology. PCR products containing "difficult-to-clone" sequences like AT- or GC-rich regions, toxic genes, nucleotide repeats, or strong promoters are often rearranged or lost entirely.
The p Silencer adeno 1.0-CMV System combines the adenoviral vectors with an efficient siRNA expression system. The system provides efficient siRNA delivery to numerous cell types, including many primary and other difficult to transfect cells.
New BD Living Colors subcellular localization vectors pHcRed1-Nuc and pHcRed1-Mito express the far-red fluorescent protein HcRed1 fused to a localization tag. Far-red shifted fluorescence easily distinguishes HcRed1 from other fluorescent proteins.
CopyControl BAC Cloning Kits enable the making libraries of stable, single-copy BAC clones that can be induced to high-copy number. A 1 ml culture of an induced clone yields microgram amounts of BAC DNA for fingerprinting and sequencing.