by Don Monroe, PhD The US Food and Drug Administration reported in January what it called a rebound in approval of innovative drugs in 2003.
The Z'-LYTE assay platform (Invitrogen Corp.) provides a fluorescence-based biochemical
Miltenyi Biotec has announced the release of several new MACS® products for the isolation of untouched human B cells.
Anti-human TCR V?12.1 monoclonal antibody is for validated applications including flow cytometry, immunoprecipitation, frozen tissue staining, and T cell activation. Anti-human TCR V?6.
Phospho-ERK1/ERK2 DuoSet® IC ELISA Development System kits (R&D Systems) provide an alternative to Western blots for intracellular factors involved in signal transduction, apoptosis, and transcription.
ProteomeLab IgG purity/heterogeneity assay (Beckman Coulter) is offered in kit format with a validated standard operating protocol (SOP).
BioFX's chemiluminescent substrates are used in immunoassay and immunoblotting applications. The reagents are intensified and the signal duration increased by proprietary enhancers.
HDAC8 Colorimetric/Fluorometric Activity Assay/Drug Discovery Kit is HTS-friendly assay and allows real-time monitoring of HDAC activity. This is made possible by HDAC-TRAC substrate and buffer, which also enables a choice of either colorimetric or fluorometric detection.
HSP27 [pS82] phosphoELISA is sensitive to 1,000 cells/well, demonstrated with HeLa cell lysates. Additional validated cell lines include THP-1, HT1080, Jurkat and 293 cells.
Pefakit® TAFI is a highly specific, 2-step chromogenic assay for the measurement of TAFI (Thrombin Activatable Fibrinolysis Inhibitor) in plasma.
Diagnostic Systems Laboratories, Inc. has launched an Inhibin B ELISA. It is specific for dimeric Inhibin B, does not require sample pretreatment, and can be fully automated on open-platform microplate systems.
The c-Kit proto-oncogene encodes a 145-165 kDa transmembrane receptor (CD117) with tyrosine kinase activity that is structurally related to platelet-derived growth factor. In normal tissues, monoclonal 2E4 stained mast cells and intestinal Cajal cells.
Fluoro-Jade® B is a polyanionic fluorescein derivative that sensitively and specifically binds to degenerating neurons. It is a dark red powder that has a green iridescence with excitation peak at 480 nm and emission peak at 525 nm.
New series of ELISAs and Instant ELISA kits is offered for the detection of soluble co-stimulatory molecules. The products are useful for research into how manipulation of of the co-stimulatory signals enhance or terminate immune responses.
Murine Monoclonal Antibody recognizes 8-hydroxy-deoxyguanosine, 8-hydroxy-guanine and 8-hydroxy-guanosine. The antibody can be used in ELISA and immunohistochemistry to detect these markers of oxidative damage to DNA and RNA.