by Elizabeth Tolchin At the University of Texas, M.D. Anderson Cancer Center, gene therapy is taking many forms, with researchers testing a variety of novel approaches, several of which are advancing to clinical trial. Current studies include metastatic lung cancer, head and neck cancer, and brain cancer.
by Elizabeth Tolchin Using real-time polymerase chain reaction (PCR) and gene expression assays, researchers identified six genes associated with survival in diffuse large B cell lymphoma (DLBCL), the most common form of non-Hodgkin's lymphoma. The system offers a method for molecular profiling that could be used to identify patients who would be candidates for clinical studies or for new treatment regimens.
Endothelial cell migration is critical in the vascularization cascade underlying many normal and pathological processes, such as wound healing and tumor progression. Traditional angiogenesis assays using microporous membranes involve fixing, staining, and counting migrated cells.
Murine Cytokine Biochip enables multiplexed analysis of thirty mouse cytokines, chemokines and growth factors using minimal sample volumes.
TekBench liquid handling systems are offered for cell-based assays including ELISA, ADME/TOX, Plasmid Prep, and RNA/DNA Isolation. Within a compact 3× 5 footprint, each fully enclosed system contains storage for over 1,000 microplates, containers and consumables (equal to five carousels).
Biodegradable Chitosan MatrixKits are optimized for 3D culture of specific cell types. Four types are currently available: Chondrocytes, Hepatocytes, Neuronal and Fibroblasts.
Disruptor Genie provides multi-directional action, which simultaneously agitates and vortexes at high speed, dramatically increasing cell disruption or sample re-suspension efficiency. The product can hold up to twelve 1.5- or 2.0-ml microtubes.
Vented closures are now available for all NALGENE® sterile disposable flasks. Made of HDPE, the closures feature a 0.2 μm hydrophobic PTFE membrane that provides sterile gas exchange for shaker and suspension cell culture.
Two new LiquiChip bead-based xMAP assay kits facilitate research in drug discovery, cell signaling, oncogenesis, apoptosis, and immune disorders. Optimized immobilized substrates enable detection of a wide range of Tyr and Ser/Thr kinase activities without the need for radioactivity.
Recombinant Mouse CD27 Ligand/TNFSF7 is available from R&D Systems. CD27 Ligand, also known as CD70, is a type II transmembrane glycoprotein belonging to the TNF superfamily (TNFSF). CD27 Ligand interacts with its receptor, CD27, expressed on natural killer (NK) cells and subsets of T and B cells.
UltraClean Maxi Plasmid Prep Kit, for the purification of up to 1 mg of plasmid DNA from large-scale cultures, can process up to 500 ml of an LB broth culture and up to 250 ml of a high nutrient broth culture. The kit employs a 45-minute protocol to process one large-scale culture.
CELLCOAT® protein-coated 96 and 384 well microplates are available with Collagen Type I, Poly-D-Lysine and Poly-L-Lysine coatings. Laminin and Fibronectin coatings are available.
Stericap PLUS bottle-top filter unit is a vacuum-driven device that rapidly sterilizes up to 10 liters of tissue culture media, microbiological media, buffers, and other biological solutions. The unit features the new Millipore Express® PLUS 0.
Miltenyi Biotec MACS NH media products are offered for the quantification, expansion and differentiation of nonhematopoietic (NH) stem cells from human bone marrow.
TPP and Midwest Scientific offer a disposable culture system for high-throughput screening of process parameters and mammalian production cell lines. The small-scale bioreactor for shaker systems, which is manufactured by TPP, has a nominal volume of 50 ml with a culture volume of 1 to 40 ml.