TOPAZ Growth Chip, the first in a new line of chips specialized for generating large, diffraction-quality protein crystals, is important for structural biologists as only half of proteins crystallized by any method turn out to be useful for solving structures.
by Catherine Shaffer Treatment for tuberculosis (TB) involves a grueling course of antibiotics that can last a year or more, and there is no effective vaccine. Because the disease manifests differently in humans than in animals, there are also no practical animal models for testing new treatments or vaccines. Now a team of scientists from the University of Michigan, Ann Arbor, Mich., have created a mathematical model of this intractable bacterial...
by Catherine Shaffer Researchers at the Whitehead Institute for Biomedical Research, Cambridge, Mass., have reported in Science Express that yeast chaperone Hsp104 regulates the formation of amyloid fibers by Sup35, a translation termination factor. Sup35 converts to a prion, and the prion form aggregates into beta-sheet-rich amyloid fibers.
By Mark Terry Cells have a finite number of divisions, and at each division, chromosome ends, or telomeres, shorten. When the telomeres diminish to a certain length, a number of complex pathways are triggered that halt cell division. In cancer, though, this signaling pathway is disrupted, resulting in unrestrained cell growth.
by Catherine Shaffer Two groups of researchers, working independently and using comparative genomic methods, identified a host of genes involved in the formation of cilia and flagella. Defects in these microtubule-based structures, which humans share with all animals and many unicellular organisms such as the algae Chlamydomonas, are blamed for various disorders, such as poor sperm motility, respiratory disorders, kidney disease, and blindness. The...
by Mark Terry Researchers at the Fox Chase Cancer Center, Philadelphia, linked Alzheimer's disease (AD) to a protein that helps cancer cells resist anti-tumor drugs. The protein, ABCA2, is associated with resistance to the cancer drug estramustine. It has also been linked to myelination and nervous system development. Involved in cellular transport, ABCA2 has also been associated with cholesterol loading. A recent report suggests there may be a...
by Jill Taylor With help from a grant from the National Institute of General Medical Sciences, the MetaCyc database, a metabolic pathway database that allows researchers to visualize metabolic pathways from more than 230 organisms, will soon be expanding both its content and the supporting software tool kit to provide additional querying capabilities, and a richer set of visualizations.
by Elizabeth Tolchin Researchers identified an enzyme that plays a key role in the recognition of misfolded proteins. The finding, say the researchers, may offer a potential target for several degenerative diseases, including cystic fibrosis, diabetes, and Alzheimer's disease.
by Elizabeth Tolchin In a recent study Hoffmann-La Roche used single nucleotide polymorphism (SNP) discovery and genotyping technologies provided by ParAllele BioScience to identify genetic risk factors for type 2 diabetes.
by Mark Terry Researchers identified a relationship between the inverse expression of pRb2/p130 and vascular endothelial growth factor (VEGF) and tumor aggressiveness. Together, they may act as a biomarker for hepatocarcinoma (HCC) or liver cancer.
The Corning® 500 cm 2 Square DW Spillguard Cell Culture Dish is offered for culturing large numbers of anchorage dependent cells. Spillguard multilayer film protects against media spills and contamination during handling and transport to the incubator.
FiberCell® Systems Duet hollow fiber modules are offered for cell culture scale-up for antibody and recombinant protein production.
by Elizabeth Tolchin With so much genetic expression data being produced by microarrays in the study of cancer, knowing the extent to which these studies agree can provide valuable insight. That is just what researchers from Johns Hopkins University, Baltimore, set out to find.
Rapid Fractionation Of Complex Protein Extracts With Centrifugal MembraneAdsorber Units Improves Detection Of Proteins In 2d PageMay 19, 2004 10:51 am | Articles | Comments
Two-dimensional gel electrophoresis followed by mass spectrometry for protein identification is a reliable and reproducible means of sample analysis. A key element in this process is reducing sample complexity, as samples often consist of tens of thousands of proteins in a magnitude of different concentrations.
by Elizabeth Tolchin In a notice issued this week, the US Food and Drug Administration (FDA) announced a new guidance to encourage the development of fixed dose combinations (FDC) and copackaged products for the treatment of HIV infection and AIDS.