SprintPrep plasmid purification system, based on Agencourt's patented SPRI chemistry, enables plasmid purification in less than 15 minutes. Carboxylate-modified paramagnetic microparticles separate cellular debris and genomic DNA from plasmid DNA without centrifugation or filtration steps.
by Elizabeth Tolchin Scientists have found a new technique for performing ultra-small-volume chemical reactions. The technique uses giant (cell-sized) liposomes as nanovials and an ultraviolet laser pulse to fuse them together. The approach may be useful for faster, cheaper identification of new pharmaceuticals and for studying cellular-level processes.
by Pam Holland-Moritz Starving Escherichia coli bacteria appears to increase their ability to mutate and adapt to the situation. Spontaneous mutations are quite normal in bacteria under environmental stress, but a report published in Molecular Microbiology [J. C. Layton, P. L. Foster, vol. 50, pp. 549-561 (2003)] showed that some bacteria increase genetic mutations in response to nutrient restriction.
Confocal microscopy offers a number of advantages when compared with traditional widefield optical microscopy. Confocal systems occlude out-of-focus light, vastly improving imaging contrast.
Monoclonal antibody offered to glutamate NMDA Receptor subunits 3A and 3B recognizes both NR3A and NR3B subunits in Western Blot and immunohistochemistry applications. NR3B is the final member of the NMDA subtype of the glutamate receptor (NMDAR) family to be cloned and characterized.
New BD Living Colors subcellular localization vectors pHcRed1-Nuc and pHcRed1-Mito express the far-red fluorescent protein HcRed1 fused to a localization tag. Far-red shifted fluorescence easily distinguishes HcRed1 from other fluorescent proteins.
Apoptosis Suite enables examination of three separate biological aspects of cell death in order to assess whether each cell is in the early- mid- or late-stages of apoptosis. The suite includes assays for membrane changes (Annexin V), activation of caspase enzymes, and DNA fragmentation (TUNEL).
Magic Red substrate-based assays detect protease activity within whole living cells, and are based on a cresyl violet leaving group that fluoresces once enzyme specific peptides are cleaved.
Recombinant Human Furin is offered by R&D Systems. Furin is a member of the proprotein convertase (PC) family that belongs to the subtilisin superfamily of serine proteases.
The StressXpress Human HO-1 ELISA Kit provides a reproducible method for quantifying human Heme Oxygenase-1 in a variety of sample types. The kit detects as little as 0.78 ng/ml, and has a range of 0.78-25 ng/ml. Once samples are prepared, the assay takes less than 3 hours to perform.
Protein disulfide-isomerase (PDI) is an enzyme that in mammalian cells catalyzes post-translational thiol-disulfide exchange reactions in expressed proteins. Such exchanges lead to refolding of the protein and greatest overall protein stability. Protein folding can particularly be a problem for E.
BBMVEC are isolated from microvessels of bovine brain. Proprietary isolation technique combines with optimized growth medium to allow culturing of robust, high purity cells. The cells are cryopreserved at third passage and can be cultured and propagated for at least 16 population doublings.
DNA Repair Kits for Ku and GTBP provide fast, quantitative study of the interactions between damaged DNA and repair proteins. The DNA-binding ELISAs eliminate the need for gels, blotting and radioactivity, and are sensitive.
NPB and NPW are recently isolated endogenous ligands for GPR7 and GPR8, which are members of the G-protein coupled receptor family. GPCRs are involved in pathways that regulate growth, metabolism, reproduction and homeostasis.
Adiponectin (Acrp30), an adipocyte-specific secreted protein, circulates in plasma and plays a role in various physiological processes, such as energy homeostasis and obesity. Active recombinant human, mouse, and rat adiponectin was expressed using perspective adiponectin of cDNA.