By Catherine Shaffer - 8/03/04
Real-time PCR offers a quantitative method for viral and bacterial pathogen detection, measurement of gene expression, and mutation detection.
EPICENTRE's MasterAmp T th DNA polymerase contains both reverse transcriptase and DNA-dependent DNA polymerase activities and is offered for single-tube RT-PCR. Reverse transcription using the product can be performed at temperatures up to 60 C to significantly reduce RNA secondary structure.
UV PCR Workstation brings together UV light and antimicrobial stainless steel to create a protective environment against PCR contamination. The unit's chamber can be decontaminated between procedures with high intensity shortwave 254 nm UV.
GeneFishing Technology offers a unique approach for identifying only authentic Differentially Expressed Genes (DEGs) in two or more nucleic acid samples. This technology improves specificity of PCR amplification, enabling researchers to find only real PCR products as a result.
Plastic disposables for PCR include 0.2 ml and 0.5 ml tubes; tube and cap strips in a variety of colors; non-skirted and semi-skirted 96-well plates; skirted 384-well plates; sealing films; and more.
Six new kits are offered chemical, non-radioactive labeling of nucleic acids, including single-stranded, double-stranded, linear or super-coiled DNAg of target in a single reaction. Simple labeling protocols can be used to attach biotin, dinitrophenol, fluorescein, dGreen, rhodamine, or DEAC labels.
Spectrolinker XL-1000 UV crosslinker covalently binds nucleic acids to membranes in less than 30 seconds.
TripleMaster PCR product is suitable for long range PCR high fidelity PCR, and applications involving GC-rich or impure templates. The kit contains a Tuning Buffer to prevent long templates from breaking and to increase PCR sensitivity, resulting in PCRs up to 40 kb.
Multiply PCR plates are made from thin-walled polypropylene to optimize temperature transfer from heating block to specimen. Test plates both without, half or full lateral skirt are available on request.
The microscope-compatible workstation can now accommodate both single- and dual-ocular microscopes. This PCR Workstation protects against PCR contamination in two ways.
The THEQ LifeCycler series' combination of server and workstation modules and virtual networking capabilities allow for individual PCR workload definition with efficient centralized operation.
The Applied Biosystems 7300 Real-Time PCR System and the Applied Biosystems 7500 Real-Time PCR System combine thermal cycling, fluorescence detection, and application-specific software, offering an affordable solution for low-throughput genomic research applications.
AMPure® is a SPRI-based PCR purification system that efficiently purifies amplicons as small as 100 bp. PCR amplicons are selectively bound to polystyrene paramagnetic microparticles, thus eliminating the high background and lower yields of silica-based magnetic beads.
This DNA amplification system for real-time detection and quantification of specific cDNA and genomic DNA targets is compatible with all real-time PCR instruments and readily adapted for use in single or multiplex analyses. QZyme Assays measure fewer than ten copies of target DNA.