Kits and reagents for optimizing apoptosis detection in particular research models include proteins and antibodies to assess expression of Bcl-2 family members, survivin and IAPs and to evaluate activity of caspases, PARP and cytochrome c.
Upstate offers a rapid and sensitive microplate-based assay for the detection of H2A.X phosphorylation. Histone H2A.
MBL International has released the 2003 Apoptosis Detection and Analysis Catalog. This resource contains nearly 3 times as many products as the 2002 version, as well as updated tables, graphics, and protocols.
Caspase selective inhibitors and substrates provide critical tools for dissecting the complex mechanisms involved in apoptosis. Q-VD-OPH, a novel compound for in vivo and in vitro applications, is an irreversible broad- spectrum caspase inhibitor.
CellTiter-Blue Cell Viability Assay, a homogeneous method for estimating the number of viable
BioVision offers assays for analyzing the early, middle, and late stages in the apoptotic cascade, and for studying apoptotic events that occur in the different areas of the cells, including the plasma membrane, cytoplasm, mitochondria, and nucleus.
Chemicon® International's ApopTag® apoptosis detection kits detect DNA fragmentation, a hallmark of apoptosis. The newest kit in the line is the ApopTag Peroxidase In Situ Oligo Ligation (ISOL) Apoptosis Detection Kit.
The M30 Apoptosense ELISA, a tool for the investigation of tumor apoptosis, is specific for apoptosis in epithelial cells and has sensitivity to detect apoptosis in less than 10,000 cells.
The CellProbe HT Caspase 3/7 Whole Cell Assay Kit enables apoptosis detection. CellProbe HT assays are whole cell based and can be automated on Beckman Coulter Biomek® liquid handling workstations.
New phosphoELISA kits offered by BioSource include Akt [pT308], Akt [pS473], Bcl-2 [pS70], CREB [pS133], [pS32], JNK [pTpY183/185], p38 MAPK [pTpY180/182], STAT1 [pY701] and others.
CASPASE-3 Cellular Activity Assay Kit PLUS, from BIOMOL Research Laboratories, provides a complete
The BD Cytometric Bead Array (CBA) Human Apoptosis Kit simultaneously measures Active Caspase-3, Bcl-2, and Cleaved PARP in a single assay, providing a comprehensive view of the apoptotic status of cell or tissue lysates.
The Active Motif line of apoptosis research reagents and kits includes mono- and polyclonal antibodies directed against caspases, death receptors, DRAKs, STATs, E2Fs, tumor suppressors such as p53, p63 and p73, among others.
The APOPercentage Assay is specific for apoptotic cells (necrotic cells remain unlabelled), and is recommended for mammalian anchorage dependent cells with regular phospholipid composition.
Claudin-7, one of the 23 identified members of the claudin protein family, is a tight junction protein expressed in normal human and mouse epithelia, and down-regulated in head and neck squamous cell carcinoma.