AmpliScribe T7- Flash in vitro transcription kit produces high yields of RNA in a short reaction time. The standard 30 minute kit reaction produces 160 - 180 μg (8 -9 mg/ml) of RNA from 1 μg of DNA template. A 6-fold linear scale up produces >1 mg of RNA in just 30 minutes.
The use of RNAi as a genetic tool in Drosophila is made easier through the availability of the Drosophila RNAi Collection, a set of over 7,000 DNA constructs ready for in vitro transcription. The collection consists of dsDNAs covering 50% of the Drosophila genome.
The Lamin A/C siRNA/siAb Assay Kit provides a complete, validated assay for silencing a commonly expressed endogenous gene.
High quality, DNA free total RNAs are isolated from more than 17 distinct regions of human brains from patients suffering from Alzheimer's, Parkinson's, and AEL. All tissues are ethically obtained and trained neuroanatomists dissect the specific brain regions.
by Elizabeth Tolchin A technique that uses ultrasound energy to create microbubbles inside specially treated liposomes and then directs the liposomes to specific targets may offer a new method for targeted gene therapy and drug delivery.
by Elizabeth Tolchin A recently discovered protein plays a role in preventing cell death when DNA damage occurs. Cells with DNA damage are able to remain alive, furthering the development of cancer.
by Elizabeth Tolchin Two companies plan to work together to create a Quebec Linkage Disequilibrium Map (QLDM) consisting of approximately 40,000 single-nucleotide polymorphisms (SNPs).
by Elizabeth Tolchin Researchers demonstrated a new microarray-supported approach for developing pathway-specific assays for high-throughput screening of compound libraries.
by Elizabeth Tolchin Illumina Inc. recently announced two new Sentrix BeadChips for whole-genome expression profiling of multiple samples on a single chip. The new six-sample whole-genome BeadChip is to compete with microarray products that analyze one genome on a chip.
by Elizabeth Tolchin Experiments with chemical genetic screens for finding new drug targets led to a method that uses a yeast culture to identify the gene products that functionally interact with small molecules and inhibit cell proliferation.
by Elizabeth Tolchin A new technique called enhanced amide hydrogen/deuterium-exchange mass spectrometry, or DXMS, promises to take some of the frustration out of protein crystallization.
TB DRY is a single powder formulation of the classic Terrific Broth. It is primarily used to grow E. coli cultures harboring low copy plasmids. Specifically designed to give high yields of low copy plasmids and extremely high yields of high copy plasmids, the media is pH stabilized with Tris.
The Wheaton Bench Top Roller Culture basic unit can accommodate two vessels of varying sizes from 108 to 121 mm diameter and up to 290 mm length. If required, the basic unit can be expanded to accommodate four or six vessels by the addition of one or two, two-place deck kits.
The CARR® ViaFuge® V12 separation system provides fast, gentle, medium-to-large scale supernatant clarification and cell harvesting. A wide variety of mammalian and insect cells are rapidly separated with little to no damage, up to production scale quantities.