Method for creating more diverse tetracyclines
Promising comprehensive coverage
Protein knockout produces fat-burning mice
G protein coupled receptors (GPCRs) are a major class of targets for drug discovery and frequently are targets within high throughput screening (HTS) laboratories. (1) Although GPCRs are a large, diverse and highly conserved class of membrane-bound proteins, signaling principally involves modulation of only two membrane-bound enzymes, adenylyl cyclase and phospholipase C.
The microflex LT benchtop MALDI-TOF mass spectrometer (Bruker Daltonics) is offered for clinical proteomics and for the routine analysis of peptides, proteins and other large molecules. Due to a gridless ion source, this instrument provides sensitivity in both positive and negative ion mode.
ProteomeScan generates images of up to 12,800 × 12,800-dpi max output resolution, ensuring that small protein spots are detected.
Macroporous reversed-phase C18 (mRP-C18) high-recovery protein fractionation and desalting LC column (Agilent Technologies) enables detection and identification of potential biomarkers in complex samples, such as serum or plasma, by providing sample recovery of greater than 95%.
SPN and SPN-htp protein assays, suitable for use with as little as 0.5 μg protein, resist interference from common laboratory agents.
pIRES2-ZsGreen1 or pZsGreen1-1 fluorescent protein vectors are offered for cellular assays. pIRES2-ZsGreen1 contains an internal ribosome entry site (IRES), which permits both the cloned gene and the ZsGreen1-encoding region to be translated from a single mRNA.
Liquid Tissue MS Protein Prep Kit makes mass spectrometry analysis of formalin-fixed tissue possible.
Cloning vectors for fluorescent proteins derived from Aequorea Victoria are available from Invitrogen. These clones will enable scientists to employ Emerald Green (EmGFP), the next-generation replacement for EGFP, as well as Cyan (CFP) and Blue (BFP) Fluorescent Proteins.
A complete selection of highly specific monoclonal antibodies to the nuclear hormone receptor protein family is available. Nuclear hormone receptors are targets for drug development as many are implicated in diabetes and other endocrine disorders, as well as cancer.
Varian’s NMR (Nuclear Magnetic Resonance) System incorporates a data acquisition control platform,
A new technology for the production of recombinant baculoviruses, the flashBAC system removes the need to separate recombinant virus from parental virus by plaque purification or any other means.
HisTrap FF crude (1 ml and 5 ml) pre-packed columns enable fast, reliable and simple one-step purification of histidine-tagged recombinant proteins directly from un-clarified cell lysates. The crude columns minimize the need for sample pre-treatment.