by Chris Dickey, DrPH The productivity/innovation gap identified in a now-famous equation developed in 1996 by Juergen Drews, MD, that sent shock waves through the pharmaceutical industry is showing few signs of abating despite the addition of thousands of potential new genomic-based targets.
by Chris Dickey, DrPH Through use of high-throughput screening, scientists at Hoffmann-La Roche, Nutley, N.J., identified a class of small molecules called Nutlins, which activates the tumor suppressor p53 by preventing the binding of a key inhibitor.
by Elizabeth Tolchin Looking for a new source of venture capital for that great idea you've been carrying around? Call Novartis. The Novartis Venture Fund, which recently released its 2003 activity report, committed $35 million in new financing last year.
by Elizabeth Tolchin There are literally hundreds of small computer programs that analyze diffraction data obtained from x-ray protein crystallography. A new program, called Elves, provides a unified interface that removes the tedious human intervention involved in using those other programs.
Fluorescence resonance energy transfer (FRET) is a technique used for quantifying the distance between two molecules conjugated to different fluorophores.
GeneEraser siRNA transfection reagent is designed for transfection of siRNA into mammalian cells. This low-toxicity reagent has demonstrated high transfection efficiency of siRNA in a variety of cell lines.
T7 RiboMAX Express RNAi System (a,b,c) is an in vitro transcription system designed for producing milligram amounts of double-stranded RNA (dsRNA) in a short amount of time.
The RNA Inspector Kit evaluates RNA and cDNA for the presence of transcripts from 1 kb to 14 kb from human, mouse and rat tissues and cell lines. The kit evaluates RNA integrity and quality, the success of cDNA synthesis, and ensures full-length cDNA when constructing cDNA libraries.
Label IT® siRNA Tracker Intracellular Localization Kits directly label and deliver siRNA of individual design in an efficient, non-destructive manner, for in vitro tracking experiments.
AmpliScribe T7- Flash in vitro transcription kit produces high yields of RNA in a short reaction time. The standard 30 minute kit reaction produces 160 - 180 μg (8 -9 mg/ml) of RNA from 1 μg of DNA template. A 6-fold linear scale up produces >1 mg of RNA in just 30 minutes.
The use of RNAi as a genetic tool in Drosophila is made easier through the availability of the Drosophila RNAi Collection, a set of over 7,000 DNA constructs ready for in vitro transcription. The collection consists of dsDNAs covering 50% of the Drosophila genome.
The Lamin A/C siRNA/siAb Assay Kit provides a complete, validated assay for silencing a commonly expressed endogenous gene.
High quality, DNA free total RNAs are isolated from more than 17 distinct regions of human brains from patients suffering from Alzheimer's, Parkinson's, and AEL. All tissues are ethically obtained and trained neuroanatomists dissect the specific brain regions.
by Elizabeth Tolchin A technique that uses ultrasound energy to create microbubbles inside specially treated liposomes and then directs the liposomes to specific targets may offer a new method for targeted gene therapy and drug delivery.
by Elizabeth Tolchin A recently discovered protein plays a role in preventing cell death when DNA damage occurs. Cells with DNA damage are able to remain alive, furthering the development of cancer.