Chemically competent E.coli cell lines have been developed using a proprietary process that both improves and maintains transformation efficiencies of the cells in single-shot tubes and 96-well plates. The process has been applied to strains of BL21, DH5Alpha and JM109.
CellStar dishes are manufactured from the virgin polystyrene and offer distortion-free optical clarity. Dishes are available in 35 × 10, 60 × 15, 94 × 16, 100 × 20 and 140 × 20mm formats. Lids are vented to provide uniform gas exchange and reduce condensation.
Gibco Advanced D-MEM/F-12 and Advanced RPMI 1640 reduced serum media formulations enable researchers involved in mammalian cell culture to reduce Fetal Bovine Serum (FBS) supplementation by 50 to 90 percent with little or no change in cell growth, promotion, morphology or function.
Kimble and Kontes have upgraded all of their filter flasks, which now feature a heavier, more uniform bead for less chipping and maximum durability. Heavier walls mean less chance of breakage during vacuum filtration. Beaded bottoms increase scratch resistance.
New Brunswick Scientific has published a series of application reports on the use of their benchtop BioFlo 110 fermentor/cell culture system. Five studies document step-by-step procedures for culturing Chinese Hamster Ovary (CHO), hybridoma and insect cells, as well as E. coli and Pichia pastoris.
New 225 cm 2 NUNC EasYFlask is equipped with a system that allows opening or closing the flask by a mere one-third turn of the cap. Maximum airflow underneath the flask optimizes temperature equilibration during incubation, even when stacked.
ECIS Model 1600 (Electric Cell-substrate Impedance Sensing) measurement follows the impedance of cell-coated electrodes in real-time and is non-invasive and quantitative.
A Human ACE Quantikine( ELISA kit is available from R&D Systems. Angiotensin I Converting Enzyme is known for its role in blood pressure control and water and salt metabolism. ACE is also involved in immunity, reproduction and neuropeptide regulation due to its localization and specificity.
The entry-level Biomass Monitor Model 210 provides a real-time record of the viable cell concentration, allowing users to see those critical process events.
Accurately counting mammalian cells in under a minute, the NucleoCounter uses patented fluorescence technology and disposable cartridges pre-filled with a nuclei-staining dye to sample and analyze cell counts.
PuraMatrix Peptide Hydrogel synthetic matrix, consisting of standard amino acids and >99% water, creates defined 3-D microenvironments for a variety of cell culture experiments.
New 384 well optical imaging microplate for high content screening is manufactured using a molding process that ensures flat well bottoms and well-to-well uniformity. The dimensional stability of this plate ensures that microscope refocusing is minimized for higher throughput imaging.
ATCC has introduced E. coli competent cells and SOC Medium for researchers who utilize transformation in the study of gene expression, cloning, and other applications. GC5 and GC10 cells consistently show transformation efficiencies of >10 9 , and are are resistant to lytic phages T1 and T5.
The Enchant Life Sciences Kit for albumin depletion and the two Enchant Life Sciences Kits for IgG (immunoglobulin G) purification remove these abundant proteins from human and animal-derived serum or plasma samples.
The S4 Pipetting Workstation is a compact, multi-function tabletop pipetting station for nanoliter to microliter dispensing in 96-, 384- and 1536-well plates. The workstation can be used with fixed or disposable tips. An automatic changing protocol simplifies tip replacement.