The indicated cell lines were plated in 12-well plates and incubated overnight at 37 C. They were then transfected at approximately 80% confluency with 1.0 μg of a capped, polyadenylated in vitro transcript encoding firefly luciferase using the TransIT-mRNA Transfection Kit.
The low noise two-channel wireless data acquisition system, RatPaak, is used to monitor
The Metabolic Monitoring System measures oxygen consumption and carbon dioxide production in lab animals. It uses an open circuit calorimetry technique and is very flexible, making the size of the animal immaterial.
A range of sterilization-resistant laboratory labels are available for wet, dry and radiation sterilization processes.
The Kinematica POLYTRON PT 1200 E handheld homogenizer offers ergonomic features that minimize the stresses related with routine and repetitive homogenization in the lab. With a 100 W motor and fingertip control of speeds up to 25,000 rpm, the device processes samples as large as 250 ml.
Eppendorf twin.tec PCR plates are manufactured using “twin-shot” technology, which combines a polycarbonate plate frame for high mechanical stability and a precise seal that prevents evaporation with polypropylene wells for maximum sample recovery.
Proprietary monoclonal DJ-1 antibody is specific for human DJ-1. It is characterized for immunohistochemistry, Western blotting, Immunofluorescence and ELISA. The conserved approx.
The RM2265 fully motorized and programmable rotary microtome offers user safety, ergonomic and performance reliability. Accessible controls reduce repetitive motions.
Using the Videomicroscope, researchers can view surgical sites hands-free on a clear and color-accurate 6.4 flat panel monitor. A continuous magnification range of more than 50× makes even the small surgical procedures visible and clear.
The Cascade:1K EMCCD camera is equipped with a 1004 × 1002-pixel detection array. The camera’s megapixel detector uses “on-chip multiplication gain” to increase signal-to-noise ratio for low-light live-cell microscopy.
The EMS 002 Plunge Freezer provides a semi-automatic method for cryo-fixation of samples. Integrated into a small unit, the plunging and controlling system allows for the introduction of all common cryogens.
Manual Stereotaxic Injector provides a simple yet effective method for injecting or withdrawing calibrated volumes. Manual control allows precise infusion in volumes as small as 200 picoliters. The injector mounts easily on a Lab Standard, Digital Lab Standard or most other stereotaxic instruments.
The VLT Series Immersion Cooler series offers three redesigned low-temperature units that can be used to achieve low temperature capability with non-refrigerated circulators, or boost the cooling capacity of refrigerated circulators.
Spin- out Columns are offered for rapid processing of small samples. Researchers apply the sample on top of the column, spin briefly, and then collect the clean samples. Columns are suitable for removing salt, buffer exchange, unincorporated radioisotopes and removal of dye, primer, dt mix etc.
The monolithic silica Onyx HPLC column portfolio offers decreased column backpressure, which enables fast separations, high sample-throughput, and high separation efficiencies by column coupling. Column lifetime is increased, even with biological samples.