The indicated cell lines were plated in 12-well plates and incubated overnight at 37 C. They were then transfected at approximately 80% confluency with 1.0 μg of a capped, polyadenylated in vitro transcript encoding firefly luciferase using the TransIT-mRNA Transfection Kit.
The low noise two-channel wireless data acquisition system, RatPaak, is used to monitor
The Metabolic Monitoring System measures oxygen consumption and carbon dioxide production in lab animals. It uses an open circuit calorimetry technique and is very flexible, making the size of the animal immaterial.
Human and Mouse IFN ELISPOT Kits, as well as a Starter ELSPOT Kit, are available from Pierce Biotechnology under its Endogen brand.
Hypersil GOLD LC and LC/MS columns, based on highly pure silica, are available in 3 and 5 μm sizes, and in formats including standard analytical columns, KAPPA capillary columns, DASH high-throughput LC/MS columns, and PIONEER direct-connection columns, as well as in several guard formats.
PRO Lab Plus Series equipment includes the VSN-5 Nutating Mixer, the VSM-3 Vortex Mixer, the VSOS-4 Orbital Shaker and the VSR-50 Variable Speed Rocker. A variety of accessories facilitate instrument integration into current lab procedures.
Cayman's PGD2 Fluorescence Polarization Immunoassay (FPIA) (Prostaglandin D2 FPIA Kit - Green) is designed for High Throughput Screening (HTS) applications for the direct, rapid measurement of PGD2.
SIMAX brand borosilicate Glass Bottles are suitable for mixing, sampling and storage. A molded glass ridge indicates full capacity. Polypropylene screw cap has a drip-free pouring ring and permanent white graduations and marking areas. Group 3.
Single quadrupole LC/MS detector Finnigan Surveyor MSQ Plus offers rapid and clear mass identification for chromatographic peaks, is optimized for chromatography workflows, and measures 12 inches wide.
Programmable temperature-controlled slide processing system ThermoBrite automates the denaturation and hybridization steps in slide-based fluorescent in situ hybridization (F)ISH assays. The accepts a range of sample types.
Mouse Tie-2 Quantikine ELISA kit is available from R&D Systems. Tie-2 is a 145 kDa, type 1 transmembrane glycoprotein that functions as a ligand-induced homodimer.
Polymeric Solid Phase Extraction (SPE) materials are offered for use in sample preparation, synthesis and purification, including pre and post HPLC applications.
Centricon Plus-70 centrifugal filter device concentrates and desalts proteins, antibodies, viruses from chromatography fractions, cell culture supernatants, fermentation broths, and biological fluids. It can also be used for exchanging buffers.
Nonlinear Dynamics Ltd. has announced the latest release of their next generation Phoretix 2D analysis products, including new versions of Phoretix 2D Evolution and Phoretix 2D Expression.
Screening Sets, for development of 5 colorimetric ELISA plates each, are currently available for human IFN?, IL-1?, IL-6, IL-8 and TNF?; bovine IFN?; mouse IFN? and MCP-1; porcine TNF?; and primate IL-1? and TNF?, with more released each month.