Magic Red substrate-based assays detect protease activity within whole living cells, and are based on a cresyl violet leaving group that fluoresces once enzyme specific peptides are cleaved.
Apoptosis Suite enables examination of three separate biological aspects of cell death in order to assess whether each cell is in the early- mid- or late-stages of apoptosis. The suite includes assays for membrane changes (Annexin V), activation of caspase enzymes, and DNA fragmentation (TUNEL).
New BD Living Colors subcellular localization vectors pHcRed1-Nuc and pHcRed1-Mito express the far-red fluorescent protein HcRed1 fused to a localization tag. Far-red shifted fluorescence easily distinguishes HcRed1 from other fluorescent proteins.
Monoclonal antibody offered to glutamate NMDA Receptor subunits 3A and 3B recognizes both NR3A and NR3B subunits in Western Blot and immunohistochemistry applications. NR3B is the final member of the NMDA subtype of the glutamate receptor (NMDAR) family to be cloned and characterized.
Upstate offers a rapid and sensitive microplate-based assay for the detection of H2A.X phosphorylation. Histone H2A.
Adiponectin (Acrp30), an adipocyte-specific secreted protein, circulates in plasma and plays a role in various physiological processes, such as energy homeostasis and obesity. Active recombinant human, mouse, and rat adiponectin was expressed using perspective adiponectin of cDNA.
NPB and NPW are recently isolated endogenous ligands for GPR7 and GPR8, which are members of the G-protein coupled receptor family. GPCRs are involved in pathways that regulate growth, metabolism, reproduction and homeostasis.
DNA Repair Kits for Ku and GTBP provide fast, quantitative study of the interactions between damaged DNA and repair proteins. The DNA-binding ELISAs eliminate the need for gels, blotting and radioactivity, and are sensitive.
BBMVEC are isolated from microvessels of bovine brain. Proprietary isolation technique combines with optimized growth medium to allow culturing of robust, high purity cells. The cells are cryopreserved at third passage and can be cultured and propagated for at least 16 population doublings.
The KODAK Image Station 2000 Multi-Modal Imaging System (IS2000MM) utilizes a cooled CCD camera to provide comprehensive fluorescence, luminescence, radiographic and chromogenic imaging.
The CelljecT Uno electroporator is a compact unit designed originally for bacterial applications. A new set of protocols for yeast applications greatly expands the role of this unit. These protocols are supplied with every new unit, along with a free 40-page optimization guide.
Cloning Gun cordless, rechargeable, hand-held electroporation instrument achieves transfection efficiencies exceeding 50% of viable cells with a variety of standard mammalian cell lines, and Transformation Efficiencies (TE's) of over 10 10 transformants per μg of input DNA with E.
MicroPulser electroporator enables safe and reproducible transformation of bacteria, yeast, and other microorganisms. The system offers one-button pulse delivery, attached cuvette chamber, and rapid charge-time speed sample handling.
The BTX ECM 830 electroporator was cited in "RNA Interference Shows Critical Requirement for NF-?B p50 in the Production of IL-12 by Human Dendritic Cells," (Laderach, D., Campagno, D., Danos, O., Vainchenker, W. Galy, A. ( J. Immunology, 171:1750-1757 (2003).
Electroporation cuvettes are offered in three color-coded gap sizes suitable for electroporation of bacteria, yeast or mammalian cells. The cuvettes fit most brands of electroporators and are individually wrapped and gamma irradiated for sterility.